Seroneutralization of Porcine Reproductive and Respiratory Syndrome Virus Correlates with Antibody Response to the GP5 Major Envelope Glycoprotein
نویسندگان
چکیده
منابع مشابه
Variations in the major envelope glycoprotein GP5 of Czech strains of porcine reproductive and respiratory syndrome virus.
The major envelope glycoprotein genes (ORF5) of seven Czech isolates of porcine reproductive and respiratory syndrome virus (PRRSV) were amplified and their nucleotide sequences were determined. ORF5 displayed nucleotide and amino acid identities of 87.5-100% and 87. 6-100%, respectively, among the isolates. In a phylogenetic tree, all European isolates were grouped in a genotype distinct from ...
متن کاملIdentification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain.
After infection of swine with porcine reproductive and respiratory syndrome virus (PRRSV), there is a rapid rise of PRRSV-specific nonneutralizing antibodies (NNA), while neutralizing antibodies (NA) are detectable not sooner than 3 weeks later. To characterize neutralizing epitopes, we selected phages from a 12-mer phage display library using anti-PRRSV neutralizing monoclonal antibody (MAb) I...
متن کاملPurification of the major envelop protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of an economically important swine disease that has been devastating the global swine industry since the early 1990s. The current PRRSV vaccines are not very effective largely due to heterogeneic nature of the virus. The major envelope protein, GP5, exposes outside the virion, induces neutralizing antibodies, and thus is a...
متن کاملThe major envelope protein, GP5, of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain.
A set of neutralizing monoclonal antibodies (mAbs) directed against the GP(5) protein of European type porcine reproductive and respiratory syndrome virus (PRRSV) has been produced previously (Weiland et al., 1999). This set reacted with a plaque-purified virus (PPV) subpopulation of Dutch isolate Intervet-10 (I-10), but not with the European prototype PRRSV LV. In order to map the neutralizati...
متن کاملSignificance of the oligosaccharides of the porcine reproductive and respiratory syndrome virus glycoproteins GP2a and GP5 for infectious virus production.
The arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) contains four glycoproteins, GP(2a), GP(3), GP(4) and GP(5), the functions of which are still largely unresolved. In this study, the significance of the N-glycosylation of the GP(2a) and GP(5) proteins of PRRSV strain LV was investigated. Both glycoproteins contain two predicted N-glycosylation sites that are highly con...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 1999
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063879901100103